FastQCFastQC Report
Tue 24 Mar 2026
SAMPLE_A_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSAMPLE_A_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8
Total Bases600 bp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTGCTTAGTATGTATTAGGCCACCATAGATCCCGTTCTGATAGTTTT112.5No Hit
ATAGTAAATTGCATAGTTGAATTTTTTACTGATCGTACATAGAGTGCAGG112.5No Hit
ACAGAGGTGGATACAATGATCTTTGCTTAGTCACTTCTGATTTGGGTCAT112.5No Hit
CGGCATAAGATTATTTTTACCTGGAAGTGATAGCCTTACGATTCAGTATT112.5No Hit
GCGTGGGACACGCGTCACACAGGTTACTTCCAAACCCCCGTGGCCCTAAG112.5No Hit
CCGTCTTCGAAAATCTGGGATCAGCAACTCGCCTTAGGCGAGGTCATGGT112.5No Hit
TGCTGCTTAGTATGTATTGGGCCACCATAGATCCCGTTCTGATAGTTTTA112.5No Hit
CTACACAGCGGACATCTCTAGGAACTAGCGACACAGGAGGGAAACGTATG112.5No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)