FastQCFastQC Report
Fri 26 Jun 2026
CMQUCU39-401_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCMQUCU39-401_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000
Total Bases150 kbp
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTTTAACCTGCTGCATATAGTCCTTCGTCTGCCAACGGATATAGAGC20.2No Hit
TGTAAGTATAAGCGCATGTCATTCATGGTAAAGGAAAGGATCACAAATAA20.2No Hit
ACAGGGCTAATGTGCTGTGAGCGATCACGCCCCCCAATCTCTCGACCTTG20.2No Hit
ATAAGGGTTCGCTTGTATCCGGACAATGATAGACGTGATTCTCTCAGCTC20.2No Hit
GCACGTCGAGCTACAACGCCTCCGATTGTAAATGCTGTCCATACTGCATA20.2No Hit
TGATTTGCCGCATTGCGCGCAGTAACGTTCGCTGCCTAGTAGGCCGTATT20.2No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)