FastQCFastQC Report
Tue 30 Jun 2026
MGB-04_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMGB-04_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82174
Total Bases20.5 Mbp
Sequences flagged as poor quality0
Sequence length250
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA30493.7104193540535935No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT28033.411054591476623No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT27453.3404726555844912No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA20582.5044417942414876No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT18772.284177477060871No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT17952.1843892228685475No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA17702.1539659746391804No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT17212.0943364081096214No Hit
CCTACGGGCGGCTGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA15341.8667705113539563No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT15251.855818141991384No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA14961.8205271740453184No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT14901.8132255944702704No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14241.7329082191447416No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT13551.648940054031689No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA10721.304548884075255No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT10251.2473531774040452No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA9721.182855891157787No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA9141.1122739552656558No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA9081.1049723756906076No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT8631.0502105288777472No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8491.0331735098693018No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT8100.9857132426314893No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT8030.9771947331272666No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA7720.9394699053228515No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT7430.9041789373767859No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA7130.8676710395015454No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA7060.8591525299973227No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6760.8226446321220824No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA6640.8080414729719863No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA6570.7995229634677634No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC6170.7508457663007764No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT5980.7277240976464575No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC5870.714337868425536No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA5860.7131209384963614No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA5240.6376712828875314No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA5090.6194173339499112No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT4890.5950787353664176No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA4870.5926448755080683No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4680.5695232068537494No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT4660.5670893469954No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA4600.559787767420352No Hit
CCTACGGGCGGCAGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA4600.559787767420352No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT4440.5403168885535571No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA4440.5403168885535571No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4410.5366660987660331No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT4290.522062939615937No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC4270.5196290797575875No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC4110.5001582008907928No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA3870.47095188259060045No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA3820.46486723294472704No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT3690.44904714386545624No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3560.4332270547861854No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC3400.4137561759193905No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA3380.4113223160610412No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC3350.4076715262735172No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3250.39550222698177034No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3250.39550222698177034No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT3250.39550222698177034No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3140.3821159977608489No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA3060.37238055832745154No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT3020.3675128386107528No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC3010.36629590868157813No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA2950.35899432910653No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2880.3504758196023073No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2720.33100494073551245No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA2720.33100494073551245No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA2680.32613722101881376No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2650.32248643123128967No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC2650.32248643123128967No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT2620.3188356414437657No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA2570.31275099179789223No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2480.3017986224353202No Hit
CCTACGGGAGGCTGCCGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA2480.3017986224353202No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2430.2957139727894468No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2400.29206318300192274No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT2350.28597853335604934No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA2320.28232774356852536No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA2230.2713753742059532No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2150.2616399347725558No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA2090.25433835519750775No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC2070.2519044953391584No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA2040.2482537055516343No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT2010.2446029157641103No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2010.2446029157641103No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1990.24216905590576093No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1950.23730133618906224No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1930.23486747633071287No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1930.23486747633071287No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1930.23486747633071287No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1920.2336505464015382No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1890.22999975661401414No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1860.22634896682649014No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC1810.22026431718061676No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1780.2166135273930927No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1750.2129627376055687No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1740.21174580767639398No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1720.20931194781804463No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1680.2044442281013459No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1680.2044442281013459No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA1640.1995765083846472No Hit
CCTACGGGTGGCTGCCGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA1640.1995765083846472No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1640.1995765083846472No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1640.1995765083846472No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1620.19714264852629787No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1620.19714264852629787No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1610.19592571859712318No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1590.1934918587387738No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA1570.19105799888042446No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT1550.1886241390220751No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA1530.18619027916372577No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1490.18132255944702705No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1440.17523790980115364No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGCTCGTAAGAGTGAC1420.1728040499428043No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1410.1715871200136296No Hit
CCTACGGGAGGCTGCCGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1340.16306861050940688No Hit
CCTACGGGAGGCTGCCGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1320.1606347506510575No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1310.15941782072188285No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC1300.15820089079270816No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA1290.15698396086353347No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1260.15333317107600944No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1220.14846545135931072No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA1200.14603159150096137No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1200.14603159150096137No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1160.14116387178426268No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA1140.1387300119259133No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1120.13629615206756396No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1070.13021150242169055No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1060.1289945724925159No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT1060.1289945724925159No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC1030.12534378270499183No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1030.12534378270499183No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1020.12412685277581716No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1010.12290992284664248No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA980.11925913305911846No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGCTCGTAAGAGTGAC970.11804220312994379No Hit
CCTACGGGAGGCTGCCGTGGGGGATATTGCACAATGGGGGAAACCCTGAT970.11804220312994379No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC960.1168252732007691No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC950.11560834327159443No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA940.11439141334241974No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT930.11317448341324507No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC910.11074062355489571No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC910.11074062355489571No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT900.10952369362572104No Hit
CCTACGGGTGGCTGCCGTGGGGGATATTGCACAATGGGGGGAACCCTGAT900.10952369362572104No Hit
CCTACGGGTGGCTGCCGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA900.10952369362572104No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA890.10830676369654635No Hit
CCTACGGGTGGCAGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT890.10830676369654635No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT870.10587290383819699No Hit
CCTACGGGAGGCTGCCGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA870.10587290383819699No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC870.10587290383819699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)