FastQCFastQC Report
Tue 30 Jun 2026
MGB-08_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMGB-08_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90527
Total Bases22.6 Mbp
Sequences flagged as poor quality0
Sequence length250
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA67227.425408994001789No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA45315.00513658908392No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT43664.822870524815801No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA39394.351188043346184No Hit
CCTACGGGCGGCTGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA32683.6099727153225007No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT30443.362532725043357No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT25672.835618102886432No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT23522.598119897931004No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA20892.30759883791576No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA14841.639289935599324No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT13371.476907441978636No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT13221.4603377997724436No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA12201.3476642327703336No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA10901.204060666983331No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA10861.1996420957283462No Hit
CCTACGGGCGGCAGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA10211.1278403128348449No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC9981.1024335281186828No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9941.098014956863698No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC9781.0803406718437594No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT9191.015166745832735No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA8310.9179581782230715No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT7720.8527842522120472No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA7650.8450517525158241No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC7250.8008660399659769No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT7250.8008660399659769No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA7230.7986567543384846No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7170.7920288974560076No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA7160.7909242546422615No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT6870.7588896130436223No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC6500.7180178289350139No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA6440.7113899720525368No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA6390.7058667579838059No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA6340.700343543915075No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA5910.6528439029239895No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC5850.6462160460415125No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC5670.6263324753940813No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA5470.6042396191191578No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC5220.5766235487755034No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC5150.5688910490792802No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT4930.5445889071768644No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT4590.5070310515094943No Hit
CCTACGGGAGGCTGCCGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA4350.48051962397958625No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA4190.4628453389596474No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT4100.45290355363593177No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA4080.4506942680084395No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA3910.4319153401747545No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3910.4319153401747545No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT3830.42307819766478505No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA3720.4109271267135771No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3420.3777878423011919No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3340.3689506997912225No Hit
CCTACGGGTGGCTGCCGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA3260.3601135572812531No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA3220.3556949860262684No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3180.3512764147712837No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGCTCGTAAGAGTGAC3160.34906712914379134No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC3070.33912534382007575No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC3020.3336021297513449No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2930.3236603444276293No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA2820.31150927347642143No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2790.30819534503518287No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA2740.302672130966452No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA2500.2761607034365438No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2490.27505606062279764No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2400.26511427529908205No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2380.2629049896715897No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC2370.2618003468578435No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGCTCGTAAGAGTGAC2300.2540678471616203No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA2290.2529632043478741No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2290.2529632043478741No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC2230.2463353474653971No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT2190.24191677621041235No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2040.22534713400421974No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGCGCAATGCTCGTAAGAGTGAC2020.22313784837672737No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA2010.22203320556298123No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1970.2176146343079965No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA1940.21430070586675795No Hit
CCTACGGGCGGCTGCCGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA1880.20767284898428096No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1860.20546356335678856No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGCGCAATGCTCGTAAGAGTGAC1850.20435892054304242No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1850.20435892054304242No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1810.1999403492880577No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1810.1999403492880577No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1680.18557999270935743No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1630.18005677864062655No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1600.176742850199388No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1580.17453356457189567No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1580.17453356457189567No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1570.1734289217581495No Hit
CCTACGGGTGGCAGCCGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA1520.16790570768941862No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1470.16238249362068774No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC1460.16127785080694157No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1450.1601732079931954No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1430.15796392236570306No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1420.1568592795519569No Hit
CCTACGGGTGGCAGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1410.15575463673821072No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1370.151336065483226No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA1320.14581285141449513No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1260.13918499453201807No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA1240.1369757089045257No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1210.1336617804632872No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA1190.13145249483579485No Hit
CCTACGGGAGGCTGCCGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1180.13034785202204868No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1160.1281385663945563No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1160.1281385663945563No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC1160.1281385663945563No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1100.12151070951207928No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA1090.12040606669833308No Hit
CCTACGGGTGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA1010.11156892418836369No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGCTCGTAAGAGTGAC1010.11156892418836369No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT1000.11046428137461752No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT990.10935963856087134No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC990.10935963856087134No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC980.10825499574712517No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA980.10825499574712517No Hit
CCTACGGGGGGCTGCCGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA970.10715035293337898No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC960.10604571011963282No Hit
CCTACGGGAGGCTGCCGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA960.10604571011963282No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT950.10494106730588665No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA950.10494106730588665No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT920.10162713886464811No Hit
CCTACGGGAGGCAGCCGTAGGGAATTTTCGTCAATGGGCGCAAGCCTGAA920.10162713886464811No Hit
CCTACGGGAGGCTGCCGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA910.10052249605090194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)